Patients characteristics are summarized in Table 2. All patients signed informed consent and Local Ethical Committee authorized the study (#2014/003,601). Implications of all the available evidenceĬSF samples were obtained from incoming patients undergoing the diagnostic lumbar puncture and enrolled at Neurological Clinic (University of Florence, Italy). Through network analysis to evaluate T-cell receptor similarity architecture, we detected a different repertoire structure in one active progressive Multiple Sclerosis patient, characterized by a high sequence similarity: in the future, it would be worthwhile to deepen this finding in progressive Multiple Sclerosis patients. We found that cerebrospinal fluid shows more public clones across repertoires, whereas peripheral blood repertoires show higher overlap with public T-cell receptor databases (McPAS-TCR and VDJdb). We found that clone distribution and prevalence were similar between Multiple Sclerosis cerebrospinal fluid and peripheral blood, but distinguished the T-cell receptor repertoire of Multiple Sclerosis patients and healthy donors. We overcome these limits by performing a statistical analysis on pooled sequences carefully checked for quality. The Lancet Regional Health – Western PacificĬoncerning T-cell receptor repertoire investigation, the use of different bioinformatics approaches and the small sample size of studies performed so far in Multiple Sclerosis cerebrospinal fluid, have hampered the identification of relevant information that may bridge molecular data and disease pathological mechanisms.The Lancet Regional Health – Southeast Asia.The Lancet Gastroenterology & Hepatology.
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